THE FITNESS OF A GEM STRAIN OF Pseudomonas aeruginosaBACTERIA COMPARING WITH ITS PARENT STRAINS UNDER LABORATORY CONDITIONS

The fitnessof a genetically engineered microorganism strainthat constructed as transconjugant by conjugation mechanism of horizontal gene transfer in Pseudomonas aeruginosa bacteria comparing with its parent strains has been assessed. Ecological fitness expressesinteractionof an organism environment. This consider good indicator for the assessment Genetically Engineered Microorganism (GEM). When released into environment this assay was carried out under laboratory condition to investigate effects environmental on fitness. These included effect differentcations(Na+, Ca+2, and Fe+3), different incubation temperatures, pH values ratios two parents. Increasing mono, di cations (Na+, Ca+2) concentrations revealed increasing parents (GEM( strain up maximum concentration (105 mM) Na+ (85mM) Ca+2 then began decrease.In three temperature plays important role rate fitness, There direct relationship between until it reaches optimum transfer. Also, highest via appeared when ratio donor recipient 3:1.The best results bacterial parentsappeared at 9, which means increased both them.These show that can be have survive well some ecological factor are similar strains.